Efficacy of the Immobilized Kocuria flava Lipase on Fe3O4/Cellulose Nanocomposite for Biodiesel Production from Cooking Oil Wastes

The increasing global demand for petroleum oils has led to a significant increase in their cost and the search renewable alternative waste resources biodiesel synthesis production using novel environmentally sound acceptable methods. In current study, Kocuria flava lipase was immobilized on Fe3O4/cellulose nanocomposite; used as biocatalyst conversion of cooking oil wastes into through transesterification/esterification process. characterization nanocomposite revealed several functional groups including carboxyl (C=O) epoxy (C-O-C) that act multipoint covalent binding sites between consequently immobility stability. showed high thermo-stability it retained about 70% its activity at 80 °C after 30 min. kinetics Km Vmax values were 0.02 mM 32.47 U/mg protein, respectively. Moreover, stability reusability reactions up four cycles with slight decline enzyme activity. Furthermore, produced characteristics compatible standards, indicating obtained is doable may be utilized our daily life diesel fuel.